IN VITRO ANTIFUNGAL ACTIVITY OF CURCUMIN MEDIATED BY PHOTODYNAMIC THERAPY ON Sporothrix brasiliensis.

Cells from both forms of the fungus were treated with pure curcumin (PDT-CUR). For this, CUR concentrations ranging from 0.09 to 50 μM were incubated for 15 minutes and then irradiated with blue LED at 15 J/cm². Similarly, it was performed with PDT-CUR-gel, at lower concentration with fungistatic action. After, a qualitative and quantitative (colony forming units (CFU)) analysis of the results was performed. Additionally, reactive oxygen species (ROS) were detected by flow cytometry. Results PDT with 0.78 μM of CUR caused a significant reduction (p < 0.05) in cells of the filamentous and yeast form, 1.38 log and 1.18 log, respectively, in comparison with the control. From the concentration of 1.56 μM of CUR, there was a total reduction in the number of CFU (≥ 3 log). The PDT-CUR-gel, in relation to its base without CUR, presented a significant reduction (p < 0.05) of 0.83 log for the filamentous form and for the yeast form, 0.72 log. ROS release was detected after the PDT-CUR assay, showing that this may be an important pathway of death caused by photoinactivation. Conclusion PDT-CUR has an important in vitro antifungal action against S. brasiliensis strains in both morphologies.