Conformational comparisons of Pasteurella multocida types B and E and structurally related capsular polysaccharides.

Glycobiology(2023)

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摘要
Pasteurella multocida, an encapsulated gram-negative bacterium, is a significant veterinary pathogen. P. multocida is classified into five serogroups (A, B, D, E, and F) based on the bacterial capsular polysaccharide (CPS), which is important for virulence. Serogroups B and E are the primary causative agents of bovine hemorrhagic septicemia that is associated with significant yearly losses of livestock worldwide, primarily in low- and middle-income countries. P. multocida disease is currently managed by whole-cell vaccination, albeit with limited efficacy. CPS is an attractive antigen target for an improved vaccine: CPS-based vaccines have proven highly effective against human bacterial diseases and could provide longer-term protection against P. multocida. The recently elucidated CPS repeat units of serogroups B and E both comprise a ManNAcA/GlcNAc disaccharide backbone with Fruf side chain, but differ in their glycosidic linkages, and a glycine side chain in serogroup B. Interestingly, the Haemophilus influenzae types e and d CPS have the same backbone residues. Here comparative modeling of P. multocida serogroups B and E and H. influenzae types e and d CPS identifies a significant impact of small structural differences on both the chain conformation and the exposed potential antibody-binding epitopes. Further, Fruf and/or glycine side chains shield the immunogenic amino-sugar CPS backbone-a possible common strategy for immune evasion in both P. multocida and H. influenzae. As the lack of common epitopes suggests limited potential for cross-reactivity, a bivalent CPS-based vaccine may be necessary to provide adequate protection against P. multocida types B and E.
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关键词
antigen conformation, capsular polysaccharide, cross reactivity, molecular modeling, Pasteurella multocida
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