Decreasing the flexibility of the TELSAM-target protein linker and omitting the cleavable fusion tag improves crystal order and diffraction limits.

Parag L Gajjar,Maria J Pedroza Romo,Celeste M Litchfield,Miles Callahan,Nathan Redd,Supeshala Nawarathnage,Sara Soleimani,Jacob Averett, Elijah Wilson,Andrew Lewis,Cameron Stewart,Yi-Jie J Tseng,Tzanko Doukov,Andrey Lebedev,James D Moody

bioRxiv : the preprint server for biology（2023）

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摘要

We demonstrate that the TELSAM protein crystallization chaperone can enable facile protein crystallization and high-resolution structure determination. We provide evidence to support the use of short but flexible linkers between TELSAM and the protein of interest and to support the avoidance of cleavable purification tags in TELSAM-fusion constructs.

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