Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation.

Dominique C Stephens,Margaret Mungai, Amber Crabtree,Heather K Beasley,Edgar Garza-Lopez, Larry Vang,Kit Neikirk,Zer Vue,Neng Vue,Andrea G Marshall,Kyrin Turner,Jian-Qiang Shao, Bishnu Sarker,Sandra Murray,Jennifer A Gaddy,Jamaine Davis,Steven M Damo,Antentor O Hinton

bioRxiv : the preprint server for biology（2023）

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摘要

Myoblasts are isolated from mouse limb muscles. They are plated for immunofluorescence-based validation using confocal microscopy. This method demonstrates the need for reliable antibodies to correctly determine and differentiate between cellular populations within isolated skeletal muscles.

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