Diagnostic performance of faecal and tissue multiplex qPCR IS900/F57 for the detection of Mycobacterium avium subspecies paratuberculosis in cattle

Francisco Jurado-Martos,Fernando Cardoso-Toset,Carmen Tarradas,Ángela Galán-Relaño,José María Sánchez-Carvajal,Inés Ruedas-Torres,Eduardo Vera,Fernanda Larenas-Muñoz,Lidia Gómez-Gascón,Irene Magdalena Rodríguez-Gómez,Librado Carrasco,Jaime Gómez-Laguna,Belén Huerta Lorenzo,Inmaculada Luque

Research in Veterinary Science（2023）

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摘要

Mycobacterium avium subspecies paratuberculosis (MAP) is responsible for bovine-paratuberculosis (bPTB), which causes high production losses in cattle. A cross-sectional study was conducted in 228 cattle to evaluate the validity and diagnostic utility of a multiplex real-time PCR (qPCR) on faecal and intestinal samples [ileocaecal valve (ICV) and ileocaecal lymph nodes (ICLN)], using intestinal tissue culture as a reference test.

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关键词

Diagnosis, Paratuberculosis, qPCR, IS 900, F57

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