A fluorescence point-of-care testing system for sample-to-answer detection of immunoglobulin E

•Fluorescence detection of proteins on cloth is firstly proposed.•Non-woven cloth is firstly used to make the test strip.•A smartphone is used to capture images and display the results via an APP.•The point-of-care testing system can achieve the sample-to-answer detection.•The IgE levels in complex human serum samples can be measured.