Comprehensive characterization of Dactylorhin and loroglossin in Dactylorhiza hatagirea D. DON using ultra-performance liquid chromatography-mass spectrometry

Dactylorhiza hatagirea D. Don is acclaimed for different medicinal properties but a little is known about its secondary-metabolites. The purpose of this study was to reveal the components of D. hatagirea by UPLC−ESI/TQ-MS approach. Metabolite profiling of D.hatagirea is reported for the first time for rapid identification of bioactive components. Molecular mass of twelve compounds were determined from LC-MS analysis of the different fractions and structures of five compounds were elucidated with MS/MS fragmentation pattern analysis. Dactylorhin C (2), B (3), A (5), and Loroglossin (4) were derivatives of 2,3-dihydroxy-2-(2-methylpropyl)butanedioic acid and 2-hydroxy-2-(2-methylpropyl)butanedioic acid. In addition, Sucrose (1) was also identified. The obtained results confirmed the presence of Dactylorhins, Loroglossin and Sucrose which have already been isolated from the plant.