Preparation of an aptamer electrochemical sensor for the highly sensitive detection of glioma cells

An aptamer-based electrochemical sensor was assembled to detect glioma cells (GC). In order to improve the detection of low concentration GC by the sensor, DNA walker was used in the setting of the sensor. The sensor can distinguish low concentration GC through the catalytic reaction caused by DNA walker. After optimizing different parameters, the proposed sensor can achieve linear detection of 10–10,000 cells/mL. The detection limit can reach 5 cell/mL. The specificity, reproducibility and stability of the biosensor were investigated. The results show that the biosensor can detect the target cells specifically and has good reproducibility. The biosensor can be stored for a long time and has no obvious effect on the detection results. Finally, the clinical application ability of biosensor was preliminarily studied by recycling test. The recovery rate and RSD of the biosensor for different concentrations of GC are within the acceptable range, which proves that the biosensor has the potential to detect clinical samples.