Development of spray dried functional milk protein concentrate containing whey proteins as fibrils

Fibrillated milk protein concentrate (MPC) was produced by selective fibrillation of whey proteins and spray dried to extend shelf life. The fibrillated MPC powder was characterized for whey protein fibrils and functionality. Observations from Thioflavin T fluorescence value, transmission electron microscopy, gel electrophoresis, rheology, and turbidity confirmed the presence of fibrils in fibrillated MPC. However, fibrils in reconstituted fibrillated MPC were thinner compared with the fibrils before spray drying. Ag-gregation of fibrils was also seen in fibrillated MPC dispersions. Further, rheological analysis of recon-stituted fibrillated MPC at different total solids levels showed that reconstituted fibrillated MPC has significantly higher viscosity, consistency index, and shear thinning behavior compared with control. Reconstituted fibrillated MPC also showed significantly lower surface tension and interfacial tension, with higher emulsification capacity, foaming capacity, and foam stability. Fibrillated MPC powder can be used as a functional ingredient in dairy and food formulations.& COPY; 2023 Elsevier Ltd. All rights reserved.