A SERS bioassay based on vancomycin-modified PEI-interlayered nanocomposite and aptamer-functionalized SERS tags for synchronous detection of Acinetobacter baumannii and Klebsiella pneumonia (vol 423, 136242, 2023)

•An aptasensor was developed to detect pathogens in food and clinical samples.•Synchronous detection of K. pneumoniae and A. baumannii was achieved.•The limit of detection was 10 cells/mL with a liner range of 50–105 cells/mL.•The total assay time was 1.5 h which is shorter compared to other diagnostic platforms.•The proposed method is a promising tool for food safety and pneumonia diagnosis.