Study on the differential proteins in plasma of the BPD sensitive to dexamethasone therapy

Objective To find some plasma differential proteins in Bronchopulmonary dysplasia(BPD) sensitive to dexamethasone therapy.Methods 30 cases of BPD from NICU of the affiliated Huaian first people’s hospital of Nanjing Medical University were selected,the plasma samples were collected before a 10-day course dexamethasone therapy for all objects,and the therapeutic effect was judged sooner after the therapy finished.The infants who showed significant therapeutic effect were collected the plasma samples again.Then,3 infants were picked at random from the dexamethasone sensitive infants ,whose plasma samples before and after dexamethasone therapy were analyzed by DIA,and the differential proteins were found and analyzed by bioinformatics.Results 10 of 30 cases were sensitive to dexamethasone.There are 23 differential proteins between two groups(before and after dexamethasone administration) in total, from which, 27 proteins are downregulated,while 6 upregulated. Bioinformatics analysis showed that differential proteins may involve multiple genes and signal pathways..When combined with literature retrieval,both low-density lipoprotein receptor-related protein 1(LRP1) and S100A8 might be consindered as potential targets for dexamethasone intervention of BPD.Conclusion We found the differential proteins related to the sensitivity to glucocorticoids for BPD, which will provide the direction for the choice of glucocorticoids in the treatment of BPD .