NIR Laser Responsive Nanoparticles for Ovarian Cancer Targeted Combination Therapy with Dual-modal Imaging Guidance

Abstract Background: Multifunctional nanoparticles with targeted therapeutic function and diagnostic-imaging are of great interest in the domain of precision therapy. The synergetic photothermal therapy (PTT)/chemotherapy with the guidance of CT/PA imaging is greatly expected to precisely kill cancer cells.Results: The synthesized FBPD NPs not only own excellent CT/PA imaging contrast increase ability but also reveal superior tumor-killing efficiency. The prepared FBPD NPs revealed excellent dispersity, great stability, outstanding optical properties. The release effciency of Dox in FBPD NPs after laser irradiation was achieved 86.7%. Meanwhile, Liquid-gas phase conversion of perfluoropentane (PFP) induced by PTT was discovered and verified, which significantly enhanced the drug release efficiency in the tumor region. Furthermore, a photosensitizer irradiated by PTT engenders heat toward tumor region. According to CCK-8 assay, the cell viability of FBPD NPs + laser group reached 8.9%. In addition, in the cytotoxicity assay in vivo, the tumors in the FBPD NPs + laser group were completely ablated. These results revealed whether in vitro or in vivo, assessments on tumor-bearing mice proved that the precise inhibiting efficiency of PTT combined with FBPD NPs, which played a significant role in the inhibition of tumor growth.Conclusion: The FBPD NPs was successfully fabricated, realizing CT/PA imaging-guided combination therapy against ovarian cancer. The unique nanoparticles with multiple abilities paves a emerging way toward precise treatment.