Usp41, a Potential Candidate Gene in Development of Breast Cancer

Abstract Background: Breast cancer is the most common cancer diagnosed among women and is the second leading cause of cancer death. It is of great significance to explore potential candidate targets. Methods: Cell function assays, siRNA, western blot, mass spectrum, flow cytometry, and other molecular biology techniques were conducted to verify the function of USP41 to breast cancer cell line. Results: The results indicate that USP41 (ubiquitin-specific proteases 41) expression is positively related to breast cancer progression. USP41 overexpression greatly enhanced breast cancer colony-forming ability, proliferation and migration. In contrast, USP41 knockdown significantly inhibited breast cancer colony-forming ability, proliferation and migration. Moreover, association of USP41 with RACK1 (Receptor for activated C kinase 1) was proved by mass spectrum, indicating its potential role in TGF-β signaling. Conclusions: USP41 can be a potential therapeutic target against breast cancer via RACK1.