Multi-omics Analysis of Redox Imbalance-Related Mild Health Problems in Monkeys

Abstract Certain symptoms associated with mild sickness and lethargy have not been categorized as definitive diseases. Confirming such symptoms in captive monkeys (Macaca fascicularis) can be difficult; however, it is possible to observe and analyze their feces. Even among monkeys that are housed under carefully monitored conditions, some monkeys occasionally have poor stool conditions. In this study, we investigated the relationship between stool state and various omics data by considering objective and quantitative values of stool water content as a phenotype for analysis. By examining the food intake of the monkeys, the stool and urine they expel, and the plasma circulating in their bodies, we attempted to obtain a comprehensive understanding of the health status of a single individual and understand its relationship with stool condition. Our metabolomics data strongly suggested an association between lipids and stool water content. Lipidomic analysis revealed the involvement of saturated and oxidized fatty acids, metallomics revealed the contribution of selenium (a bio-essential trace element), and intestinal microbiota analysis revealed the association of several bacterial species with the stool water content. Taken together, these results suggest that selenium-induced disturbance in redox balance may cause minor health problems.