Exogenous Application of Sodium Hydrosulfide Suppresses Bacterial Wilt And Regulates The Soil Microecology

Abstract The role of hydrogen sulfide (H2S) in regulating the pathogenic bacteria has been well documented. However, whether exogenous H2S addition inhibits the pathogens in soil is not understood, and whether H2S can suppress the plant disease caused by pathogen R. Solanacearum is not clear. In the present study, different concentrations of H2S donor NaHS were applied to the tobacco field to explore the interrelation among NaHS, tobacco baterial wilt, soil physicochemical properties and microbial community. In order to decipher the disease suppression mechanism from the perspective of soil microecology. Application of NaHS significantly reduced the disease incidence and disease index of TBW, increased soil pH, alkali-hydrolyzed nitrogen (AN), available phosphorus (AP), available phosphorus (AP) and organic matter (OM). NaHS addition also changed soil microbial community composition and structure. Furthermore, NaHS addition significantly reduced the abundance of Ralstonia and Fusarium, and increas pathogenic ed beneficial microorganisms Solirubrobacter, Rhodococcus, Rhizobium, Pseudomonas, Paenibacillus, Microvirga, Lysobacter, Haliangium, Granulicella, Flavobacterium, Bacillus, Trichoderma and Aspergillus at the genus level. Our findings suggested that exogenous application of NaHS significantly suppressed TBW caused by R. Solanacearum through regulated soil microecology. This study revealed the potential of NaHS in control of bacterial wilt.