Chemical toxicity screening of tire particle leachates from vehicles and their effects on organisms across three trophic levels.

Tire particles (TPs) generated on roads are a main contributor to microplastic environmental pollution. In this study, TP leachates from three vehicle types (bicycle, car, and electric scooter) were prepared. TP leachate toxicity impacts on three organisms (Vigna radiata, Daphnia magna, and Danio rerio) were analyzed, in addition to their chemical compositions. Zinc and benzothiazole were the most commonly detected compounds in all three leachate types. Growth inhibition of V. radiata, mortality of D. magna, and abnormality in D. rerio were observed as toxicological impacts. Overall, the lethal effects of TP leachates showed a significant, positive relationship with zinc and benzothiazole concentration. The results confirmed that TPs are complex contaminants, which release chemicals into the environment that affect both soil and aquatic organisms. These findings highlight the need for stricter control measures and environmental regulations to mitigate the ecotoxic effects of TPs and related contaminants across ecosystems and trophic levels.