Phytochemical Screening, HPTLC Fingerprinting and Evaluation of In Vitro Cytotoxic Activity of Stem Bark Extracts of O roxylum indicum (L.) Vent. Against Human Cervical Cancer Cells

Oroxylum indicum, a well-known traditional medicinal plant which is used to alleviate various kinds of diseases in Asia. The study aimed to identify bioactive compounds present in O. indicum stem bark using HPTLC technique. Further, the cytotoxic effects of the plant extracts were determined against HeLa (human cervical carcinoma) cell lines. The results of the study have shown the presence of the phytoconstituents such as flavonoids, phenols, tannins and steroids. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay showed that the ethanol, methanol and water extracts of O. indicum exhibited cytotoxic effect in HeLa cell lines with IC 50 values of 119, 89.43 and 114.1 µg/mL, respectively against standard doxorubicin with IC 50 value 3.895 µg/mL. The current study suggests that the methanol extract of O. indicum may offer chemopreventive properties. However, additional research is required to isolate and characterize the specific chemical entities present in O. indicum . These studies will aid in identifying a potential lead compound that holds promise as a natural anticancer agent.