Groundwater table fluctuation: A driving force affecting nitrogen transformation in nitrate-contaminated groundwater

Groundwater table fluctuation alters the biochemical environment and nitrogen transformation, leading to pe-riodic changes in groundwater nitrate concentration. A microcosm experiment of groundwater table fluctuation was designed to explore the underlying mechanism. The dissolved oxygen (DO), bacterial community, functional gene groups and associated nitrogen transformation were investigated based on field scenario. The results showed that groundwater rising reduced the average nitrate concentration, while falling aggravated it. Owing to the variations in groundwater table, the DO concentration underwent periodic changes. Furthermore, the abundance and diversity of soil bacteria were enriched and the structure of the microbial community was altered. Nitrogen transformation biomarkers such as Arthrobacter sp., Bacillus sp., Chloroflexi sp. and Acidobacteriota sp. as well as nitrogen transformation functional genes were identified. A structure equation model was established which demonstrated that groundwater table fluctuation altered DO and the dominant bacterial abundance, which in turn modified the functional gene groups and nitrogen transformation. This study broadens the un-derstanding of how and to what extent groundwater table fluctuation affects nitrogen transformation and is vital for assessing and managing risks associated with groundwater nitrate contamination