Nucleic Acid Sequence Composition of the Oxford – AstraZeneca Vaccine ChAdOx1 nCoV-19 (AZD1222, Vaxzevria)

Abstract The vaccine ChAdOx1 nCoV-19 has been widely used, but its purity has been disputed because of rare side effects. We used Nanopore sequencing to assess the sequence and genetic purity of a vaccine dose. As we were lacking a reference sequence for the antigen cassette, we provide the obtained annotated sequence of the full vector to aid further studies on this topic. Our sample adhered to the published data, was highly pure (>99.97%), and no copy of the E1 gene as a predictor on replication-competent escape mutants was found.