FindingNemo Library 1: Modified ULK001 v1

This sub-protocol is designed to prepare library from extracted ultra-high molecular weight (UHMW) DNA to obtain ultra-long (UL) reads on Nanopore sequencers. The UL library protocols we tested are based on ONT's rapid kit, i.e., SQK-ULK001, a transposase based adapter ligation kit. Modified ULK001 protocol consistently produced N50 > 100 kb from a good input quality of UHMW DNA and is our recommended route for best output as it is also the most-cost effective. Transposase-based reaction is done in a large volume of up to 1 ml. The working principle of the ULK001 protocol is shown in the diagram below: