Comprehensive Analysis of Urinary lncRNA And mRNA Expression Profiles In Patients With lupus Nephritis

Abstract Background:To explore long-non-coding RNA (lncRNAs),messenger RNAs (mRNAs) expression profiles and biological functions in the urine samples in lupus nephritis (LN) patients.Methods:Three LN patients and three healthy controls(con) were recruited,whose midstream morning urine was collected.A microarray of mRNA and lncRNA was applied to explore total RNA expression variation.Then venn analysis was applied to screen out specific gene expression in the LN group compared to the con group.Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to reveal the gene functions of the dysregulated lncRNA-associated with LN;STRING online website and Cytoscape software network analysis were applied to construct protein interaction network (PPI) and screen out the Hub Gene.Drug online websites were conducted as drug prediction websites.Results:A total of 273 mRNAs and 549 lncRNAs were differentially expressed in LN patients' urine compared with the con group.GO analysis of LN revealed that mRNAs from the lncRNA-mRNA network were enriched in terms of antigen-negative regulation of immune response.KEGG analysis of hub genes related to the LN lncRNA-mRNA network highlights their critical role in protein processing in the endoplasmic reticulum,P53 signaling pathway.Co-expression and PPI network analysis suggested that high-degree nodes are clustered in apoptosis,autophagy, reactive oxygen species pathway.Drug prediction indicated that the targeted drugs mainly included antioxidant activity,phosphorylative mechanism.Conclusion:Our findings indicated that the differential expressed urinary lncRNAs(DELs) possessed considerable clinical value in the diagnosis and potential therapeutic applications of drugs in LN patients.