Total High Molecular Weight DNA Extraction from plant tissues for Long Read Sequencing v1

This protocol is a combination of two published protocols (10.1186/1746-4811-8-26 and 10.2144/000114460) with modifications and was developed as a research within GIH collaborative projects. Using this protocol, high quality High Molecular Weight (HMW) DNA (50kb – 300kb) was obtained from a wide range of plants tissues – Neptunia (leaves & roots), Senecio (leaves) and Mangoes (Kensington pride, Alphonso, and Tommy Atkins leaves). The DNA quality was assessed in Qubit, NanoDrop, TapeStation, PFGE and Oxford Nanopore Technologies. The yielded HMW DNA is suitable for long read sequencing technologies (ONT and PacBio) or similar experiments.