Integrative Analysis of LncRNAs and miRNAs, Revealing Key Biomarkers of Condyloma Acuminata

Abstract Condyloma acuminata (CA), genital wart, is caused by HPV infections and transmitted by sexual contact. Bioinformatics analyses contributes to the discovery of hub genes and non-protein-coding RNAs of novel therapeutic relevance in CA. We screened out dysregulated miRNAs and LncRNAs in gene expression matrix of our own dataset and datasets from Arrayexpress database, GEO database and. Subsequently, miRNA- gene network and LncRNAs-miRNA interaction pair were constructed. Hub genes were identified from PPI network of target genes. It was predicted RP11-1081L13.4 and MIR31HG commonly interacted with miR-31a-5p, which is up regulated in CA. Functional enrichment analyses of GO and KEGG revealed the potential role of MAPK pathways in CA. RT-qPCR results showed that miR-31-5p was over-expressed in CA tissues. Downregulated miR-31-5p increased apoptosis and reduced proliferation and migration ability of Hela cell lines in vitro via downregulating MAPK pathway. These findings suggested that MIR31HG, RP11-1081L13.4, especially miR-31-5p and hub genes were promising biomarkers, contributing to CA pathogenesis.