A fluorescence aptasensor baesd on nanomagnetic material for sensitive and rapid detection of SMZ in animal-derived foods

Abstract A fluorescence aptasensor was developed based on two-dimensional nanomagnetic materials Fe3O4/Au/g-C3N4 and FAM-labeled aptamer of sulfamethazine (FAM-SMZ1S) for sensitive and rapid detection of sulfamethazine (SMZ) in animal-derived foods. The aptasensor exhibited a high sensitivity with the limit of detection (LOD) of 0.87 ng/mL (S/N = 3), the linear range from 1 ng/mL to 100 ng/mL. The performance of aptasensor was demonstrated in 10 real-world samples with the recovery rate in the range of 91.6%-106.82% and the coefficient of variation (CV) in the range of 2.8%-13.44%. In addition, the detection ability of the aptasensor was comparable with that of the HPLC as the detection results demonstrated good correlations (R2 > 0.9153). Therefore, the aptasensor can be a sensitive, accurate and rapid method for the detection of SMZ in animal-derived foods.