Quantum CART(qCART), apiggyBac-basedsystem for development and production of virus-free multiplex CAR-T cell therapy

AbstractChimeric antigen receptor T (CAR-T) cell therapy has the potential to transform cancer treatment. However, CAR-T therapy application is currently limited to certain types of relapsed/refractory B cell lymphomas. To unlock the full potential of CAR-T therapy, technologic breakthroughs will be needed in multiple areas, including optimization of autologous CAR-T development, shortening the innovation cycle, and further manufacturing advancement of next-generation CAR-T therapies. Here, we established a simple and robust virus-free multiplexQuantum CART™system that seamlessly and synergistically integrates four platforms: 1.GTailor™for rapid identification of lead CAR construct design, 2.Quantum Nufect™for effective but gentle electroporation-based gene delivery, 3.Quantum pBac™, featuring a virus-free transposon-based vector with large payload capacity and integration profile similar to retrovirus, and 4.iCellar™for robust and high-quality CAR+T memory stem cell expansion. This robust, virus-free multiplexQuantum CART™system is expected to unleash the full potential of CAR-T therapy for treating diseases.