Complete Amino Acid Sequences of Two γ-Thionins From Maize (Zea Mays L.) Seeds

Abstract: Two γ-thionins were purified from maize (Zea mays L.) seeds by saline extraction, ammonium sulfate precipitation, pseudo-affinity and RP-HPLC chromatographies. Their complete amino acid sequences were determined by automated Edman degradations of the intact and S-alkylated proteins and peptides obtained from proteolytic digests and confirmed by ES/MS analysis. These sequences show remarkable homology with sorghum α-amylase isoinhibtors.