miR-19b-3p inhibits glioma progression via interacting with lncRNA HAGLR

Abstract LncRNA HOXD-AS1, alias HAGLR, was reported to be a tumor promoter and involved in the induction of chemoresistance in glioma. This study was designed to infer the potential miRNA which bound with HAGLR and contributed to tumor growth in glioma. Specifically, two miRNAs (miR-17b-5p and miR-19b-3p) targeted were selected by HAGLR in glioma cells. Then we constructed miRNA-overexpression vectors and transfected into U87 cells to establish experimental cell models. CCK-8 assay was applied to examine cell viability and the cell group with miR-19b-3p upregulation exhibited a stronger inhibition on cell growth, which therefore was selected for the following experiments. Transwell assay and flow cytometry were then utilized to detect cell migration and apoptosis and dual-luciferase reporter assay was used for examining the combination of HAGLR and miR-19b-3p. The results suggested that miR-19b-3p overexpression significantly decreased cell migration but increased cell apoptosis. Moreover, upregulated miR-19b-3p considerably reduced the relative luciferase activity of the wild-type, rather than the mutant HAGLR-harboring U87 cells, indicating that HAGLR was identified as the target of miR-19b-3p. This study identified a novel HAGLR/miR-19b-3p regulation axis in cellular activities of glioma cells, which provided a preliminary theoretical basis for further research on underlying molecular mechanisms.