Oxygen Evolution Measurement: Light Curve for Algae v1

Thanks to a lovely combination of trial and error I present to you an optimized* protocol for using an oxygen electrode to create a light curve for Ostrecoccus tauri. The light curve can be used to determine what light intensity is ideal for photosynthesis in O. tauri, which can then inform the light conditions for a subsequent bicarbonate curve. An O. tauri bicarb curve could potentially indicate (when compared to other algal bicarb curves) whether or not this species has a carbon-concentrating mechanism. *This protocol could be further optimized by making a growth curve for O. tauri first. *The protocol is written for O. tauri and can be adapted to other algal species. When adapting it will be necessary to reassess optimal cell density for measurements, and consider the choice of buffer for washing and incubating cells. ASW is used in this situation but will not be suitable for fresh water species