Single-vesicle analyses validate L1CAM as a marker of neuron-derived extracellular vesicles in blood

Abstract The immunoaffinity isolation of neuron-derived extracellular vesicles (NDEVs) from blood by targeting the neuronal protein L1CAM has been widely adopted to provide peripheral biomarkers for brain disorders. However, compelling evidence for the presence of L1CAM on NDEVs at the single-EV level has been missing. Here, we demonstrate that L1CAM co-localizes with EV-characteristic and neuronal markers in plasma EVs, and quantify the enrichment achieved after L1CAM immunoprecipitation, validating the NDEV isolation methodology.