Identification of Cutibacterium acnes in spondylitis by metagenomics analysis

crossref(2022)

引用 0|浏览4
暂无评分
摘要
Abstract Background: Identifying pathogens with culture-negative pyogenic spondylitis was difficult. Shotgun metagenomic sequencing is an unbiased and culture-free approach to the diagnosis of infectious diseases. There were, however, a variety of contamination factors that confounded the precision of metagenomic sequencing. Methods: This study proposed a contamination-robust protocol for clinical metagenomic sequencing. Using this protocol, we were able to identify Cutibactierum modestum in tissue specimen of infectious spondylitis. Results: The identification of Cutibactierum modestum and annotation of its resistome were annotated. The patient recovered fully. Conclusions: This application of next-generation sequencing using the contamination-robust protocol to assist the diagnosis of Cutibactierum modestum infection provides a new perspective in the clinical approach against spinal osteomyelitis and illustrates the potential of this technique in rapid etiological diagnosis.
更多
查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要