Human Sample Processing and Isolation of Extracellular Vesicles with Size Exclusion Chromatography v1

This protocol details the steps necessary to isolate circulating plasma extracellular vesicles (EVs) from human peripheral blood samples. This protocol utilizes an automated fraction collector (AFC) and qEV size-exclusion chromatography columns from IZON Science. This is intended to serve as the first step in the workflow for EV quantification along with “Measurement of extracellular vesicles with tunable resistance pulse sensing (TRPS).”