Schistosoma mansonicercariae sexing v1

This DNA extraction method for Schistosoma mansoni cercariae is based on the HOTSHOT method https://health.uconn.edu/mouse-genome-modification/protocols/hotshot-method-of-dna-preparation/. DNA isolation is followed by PCR amplification of the "W1" female W chromosome repetitive region. The 476 bp W1 repeat was identified by Webster, Mansour, and Bieber (1989). PCR primers for the W1 repeat were designed by Gasser, Morahan, and Mitchell (1991). Existing primers for the S. mansoni actin gene are used as a positive control (Delcroix et al, 2006). W1a - 5' CAA CAC AGT GAA ATT CTT CC 3' (positions 10-29) W1b- 5' GAA TTC ACC ACT CGA CAT TC 3' (positions 463-482)