Detection of OXA-48 and NDM-1 genes in ESBL producing Ochrobactrum anthropi from urine samples of Out-patients.

Background Treatment of the urinary tract infections (UTIs) is vast becoming worrisome because prominent antibiotic resistance among the bacteria involve in the infection. Species of Ochrobacterum are also involve in the infection affecting urinary tract and profusely resistance to antibiotics. Methods Urine samples were collected from out-patients of some hospitals and the bacteria were isolated and identified based on the morphological and biochemical characteristics. The most resistant isolate was selected for molecular identification by amplifying 16S rRNA gene. The bacterial isolates were subjected to antibiotic susceptibility assay and interpreted using standard of CLSI. The resistant isolate was evaluated for the presence of ESBL genes, MBL genes, biofilm gene and efflux pump gene. Results Fourteen (14) Ochrobactrum spp. were isolated from urine samples. Only 3 (21%) of the isolates were resistance to all the twenty-one (21) antibiotics while 7 (50%) were resistance to twenty (20) antibiotics. However, resistance was observed to atleastnine (9) antibiotics in all the antibiotics by the bacterial isolates. The selected isolated was identified to be Ochrobactrum anthropic strain U0145 with occurrence of blaTEM,blaSHV and blaCTX-M-9 as ESBLs genes while blaOXA-48 and blaNDM-1 genes as MBLs genes however, there was no occurrence of GES 1-9,11 gene. The bacterium possessed biofilm production gene while there was no efflux pump gene. Conclusion O. anthropiis becoming prominent among the bacteria responsible for nosocomial-associated infection especially when catheter is involved and treatment may be tasking therefore adequate infection control should be put in place.