Impact of Diabetes Mellitus Induced in Early Postnatal Life on Bax Protein Expression in Rat Testes

The aim of our study was to investigate the impact of experimentally induced hyperglycemia on Bax expression in the testes of pubertal and mature rats.Diabetes mellitus (DM) was induced by single i.p. streptozotocin injection at dose of 100 mg/kg b.w. on day 1 (neonatally, NDM) or day 10 (prepubertally, PDM).Treated animals were sacrificed on day 18 or day 50 and testes and blood were sampled.Serum glucose was evaluated.Testis lysates were used to determine expression of pro-apoptotic Bax protein by SDS-PAGE followed by Western blot.Our results revealed prominent expression of pro-apoptotic Bax protein with most intensive reaction in adult PDM testes.Experimentally induced DM in early life increased pro-apoptotic Bax expression in pubertal and mature rat testes that is probably involved in mechanisms underlying reproductive disorders in diabetic males.