Effect of Pomegranate (Molasses, White Peel and Red Peel) extract on healthy liver and hepatotoxicity induced by Phenylhydrazine in male rats

Abstract Pomegranates (Punica granatum L.) are said to provide various health advantages, according to accumulating research. The Liver tissue and antioxidant stress effect of pomegranate molasses, white and red peel extracts on male rats with phenylhydrazine (PHZ)-induced anaemia are highlighted in this article. Phenylhydrazine induced anemia resulted in: Decreasing of Direct Bilirubin, CAT, GPx, SOD, ALP, Accompanied by increasing of LDH, Total Bilirubin, Indirect Bilirubin, MDA, ALT, AST. Regarding the histopathology study of Liver: PHZ exposed group showed a loss of the normal architecture with dilated, congested central vein and necrotic areas, dilated and congested blood sinusoids with pycnotic Kuppfer cells and hepatocytes with karyolytic nuclei. PHZ exposed group showed a severely-vacuolated hepatic environment with hydrobic degeneration apoptotic and degenerated hepatocytes, focal necrosis, dilated blood sinusoids with atrophied Kuppfer cells and dilated central vein. Regading of Molasses Pomegranate + PHZ; White Peel Pomegranate + PHZ and Red Peel Pomegranate + PHZ (Improvement Group): There was an increase of Direct Bilirubin, CAT, GPx, SOD, ALP. Accompanied by decreasing of LDH, Total Bilirubin, Indirect Bilirubin, MDA, ALT, AST, ALP. Red peel pomegranate after PHZ (Red Peel + PHZ) exposed group showed a partial restoration of the normal histological pattern to a great extent with a central vein having a good profile and hepatocytes with healthy and intact nuclei. But some vacuolization and leucocytic infiltration is still evident. White peel pomegranate after PHZ (White Peel + PHZ) exposed group showed a regeneration in hepatocytes, a nearly normal blood sinusoids, but the central vein is still congested and surrounded with damaged cells. Molasses pomegranate after PHZ (Molasses + PHZ) exposed group showed most of hepatocytes and Kuppfer cells taking normal appearance but the hepatic environment is still vacuolated (V) and the central vein is congested. Conclusion: Molasses, White peel and Red peel Pomegranate have a strong antioxidant for protective liver tissue.