The effect of photobiomodulation and Akkermansia muciniphila on THP-1 derived macrophage polarization treated with gliadin peptide

Research Square (Research Square)(2022)

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摘要
Abstract Background and objective: Photobiomodulation (PBM) and Akkermansia muciniphila (A. muciniphila)have been shown to be effective in improving inflammatory conditions with positive effects on increasing the population of anti-inflammatory M2 macrophages. In this study, gliadin stimulated THP-1 derived macrophages were treated with A. muciniphila and PBM to evaluate their effects on promoting the polarization of M2 MQs. Methods and Results: The human monocyte cell line (THP-1) was differentiated to Macrophages (MQ). MQs were stimulated with 200 μg/ml gliadin for 24 h and then were treated with PBM 810 nm alone and along with Akkermansia muciniphila for following 24 h to evaluate their effects on macrophages polarization. THP-1 derived MQs were also treated with PBM and A. muciniphila to evaluate their effects on non-stimulated MQs. CD11b, CD80, and CD206 levels were evaluated by flow cytometry technique. Moreover, the expression of some M1and M2-related cytokines were determined. PBM of gliadin stimulated MQs decreased IL-6 and increased TGF-β, IL-10 and TNF-α expression compared with gliadin exposed MQs. PBM along with A. muciniphila treatment induced IL-6, TNF-α, and IL-10 expression in MQs in comparison to the untreated group and also elevated TGF-β, IL-10 and TNF-α levels in gliadin triggered MQs related to gliadin stimulated MQ cells. Conclusion: The result of this study showed the potential of PBMT and A. muciniphila to be used for modulating inflammatory responses and macrophages polarization, which may open new perspectives to find possible therapeutic target for celiac diseases.
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macrophage polarization,photobiomodulation,peptide,akkermansia muciniphila
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