Study performance of uricase-poly (-O-methylaniline) films as bio-optode for uric acid determinations

In the present work, uricase and poly(-o-methylaniline) (UOX–PoMeANI) films was used as bio-optode development to measure uric acid (UA) in human serum of hyperuricemia or gout patients. UOX has been immobilized onto chemically synthesized PoMeANI using the absorption technique. In this developed biosensor, PoMEANI was chosen as a matrix because of its advantages. This polymer acted as a matrix as well as a redox agent and indicator due to its electrochromic effect. UOX–PoMeANI films have been characterized using spectroscopic measurement for uric acid (UA) determinations. The experimental parameters of the bio-optode were optimized. Here, the bio-optode showed a maximum response at a wavelength of 590 nm, with the optimum pH at 6 and temperature at 35oC. The calibration curve had a dynamic working range at 0.0 to 0.6 mmol/l of UA with a limit of detection (LOD) of 0.063 mmol/l UA. The bio-optode has a long lifetime in the defined condition (4oC). The bio-optode was successfully used to measure UA in human serum samples, which was shown conformity with the reference method, employing a commercially available enzyme test kit. Thus, the developed biosensor can be used as an analytical tool for uric acid determination.