Introducing uracil-containing oligo to improve exponential amplification reaction performance in biosensor application

Yuhan Deng,Zhen Zhang,Yujie Zhou,Xiaojing Pei,Yifan He,Tongbo Wu

SSRN Electronic Journal（2023）

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摘要

•The uracil-containing oligo changed the enzyme kinetics of DNA polymerase.•The uracil-containing oligo reduced non-specific amplification.•This method improved the detection limit of EXPAR.•This method enhanced the distinguishability of EXPAR.

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关键词

Exponential amplification reaction (EXPAR),Background reduction,Polymerase,Uracil-containing oilgos,Highly sensitive detection

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