Control Effect and Applicability of Bacillus Thuringiensis for Control of Lycoriella Ingenua in Agaricus Bisporus Cultivation Plots

Journal of the Faculty of Agriculture, Kyushu University(2022)

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摘要
A Bacillus thuringiensis strain, which is an entomopathogenic microorganism, was used for effective control of Lycoriella ingenua larvae, which are an intractable pest occurring during Agaricus bisporus cultivation. The effects of B. thuringiensis subsp. kyushuensis CAB464 strain that shows high insecticidal effects against L. ingenua larvae at low concentrations, which were sublethal concentrations, on the survival rate of L. ingenua larvae were reviewed and according to the results, the feeding and growth rates of the larvae were hindered, and it could be seen that while B. thuringiensis subsp. kyushuensis CAB464 strain directly acts on A. bisporus cultivation plots, it also has an insecticidal mechanism to inhibit feeding thereby starving the larvae to death. Pot tests were conducted to see the applicability and control effect of B. thuringiensis subsp. kyushuensis CAB464 strain on A. bisporus cultivation plots, and according to the results, B. thuringiensis subsp. kyushuensis CAB464 strain showed a control value of 80.1%, which was at least about 50% higher than that of the untreated control plot and showed a survival rate at least about 38% lower than that of the untreated control plot while the untreated control plot showed gradual increases in the survival rate. The control effects of B. thuringiensis subsp. kyushuensis CAB464 strain were tested in an A. bisporus cultivation plot in which L. ingenua in all age periods existed in mixture, and based on the results, the control value after two times of treatment was higher than that after one time of treatment as it was 75.6% after the first treatment and 81.7% after the second treatment, and it could be seen that the survival rate gradually decreased. The foregoing indicates that B. thuringiensis subsp. kyushuensis CAB464 strain is applicable as a novel microbial insecticide that can control L. ingenua.
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关键词
Bacillus thuringiensis, Lycoriella ingenua, microbial insecticide, mushroom cultivation
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