Phage infection and timed harvest of E. coli and B. subtilis cells v1

This protocol outlines how to infect E. coli and B. subtilis with phage T4 and SPO1, respectively, and harvest cells at set time points. Harvesting cells at different timepoints during infection allows you to evaluate when bacteriophage transcripts are most abundant in cells and maximize their enrichment for downstream analyses such as chemical analysis and RNA sequencing.