Supportive Evidence For The Potential For Novel Biodiscovery In Osteoarthritis Through The Stepup Oa Consortium

Purpose: The STEpUP OA consortium (Synovial Fluid To Define Molecular Endotypes by Unbiased Proteomics in Osteoarthritis) was set up with a primary objective to address whether OA is a single disease or made up of multiple distinct molecular endotypes, when examining large scale (SomaLogic) proteomic profiling of synovial fluid (SF) from multiple cohorts. Assay of SF as a discovery matrix using this technology (as opposed to serum/plasma) has not been previously reported other than in small sample numbers and early versions of the array. Here we present our quality control (QC) procedure and initial proof-of-concept molecular data from the STEpUP OA Discovery dataset, to test and confirm the validity of our approach.