Linking genome structures to functions by simultaneous single-cell Hi-C and RNA-seq

Zhiyuan Liu,Yujie Chen, Qimin Xia,Menghan Liu, Heming Xu,Yi Chi, Yujing Deng,Dong Xing

Science(2023)

引用 14|浏览3
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摘要
Much progress has been made recently in single-cell chromosome conformation capture technologies. However, a method that allows simultaneous profiling of chromatin architecture and gene expression has not been reported. Here, we developed an assay named “Hi-C and RNA-seq employed simultaneously” (HiRES) and performed it on thousands of single cells from developing mouse embryos. Single-cell three-dimensional genome structures, despite being heavily determined by the cell cycle and developmental stages, gradually diverged in a cell type–specific manner as development progressed. By comparing the pseudotemporal dynamics of chromatin interactions with gene expression, we found a widespread chromatin rewiring that occurred before transcription activation. Our results demonstrate that the establishment of specific chromatin interactions is tightly related to transcriptional control and cell functions during lineage specification.
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关键词
genome structures,single-cell,rna-seq
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