Production and Certification of Toxic Metal-Induced Basmati Rice: An Indigenous Cultivated Rice CRM/BND

This Bharatiya Nirdeshak Dravya, BND® 3001, is an Indian Certified Reference Material of toxic elements, viz., Lead (Pb), Cadmium (Cd), Arsenic (As) and Mercury (Hg) in Rice Flour being developed by CSIR-national physical laboratory (NPL). This indigenous certified reference material (CRM) is a trade mark of CSIR-NPL is cultivated sowing Basmati rice of PUSA 1121 and induced with toxic elements in a time interval during cultivation. The harvested rice crop has been subjected for processing through drying, separation of husk from seed, grinding, sieving and then homogenization of the rice flour material was carried out prior to bottling. The homogeneity, stability and characterization studies for the analytes in rice matrix have been carried out using CRMs and BNDs for the calibration and recovery factor estimation using atomic absorption spectroscopy (AAS), inductively coupled plasma optical emission spectroscopy and Inductively coupled plasma mass spectrometry The measurement uncertainty due to characterization was estimated according to ISO GUM Guide 100:2008 and the associated uncertainty is calculated at 95% confidence level considering the possible heterogeneity along with stability factors as per ISO Guide 35:2017. The assignment of property value of the analytes in rice flour along with its certification was done in accordance with IS/ISO 17034:2016 and ISO Guide 31:2015. BND® 3001 can be used as a primary calibration standard for the quantitative determination of Lead, Cadmium, Arsenic and Mercury and validation of method for the quantification/characterization of the measurands/analytes. BND® 3001 will ensure high quality measurements with SI traceability to the analytical results and reduce the difficulties faced by the rice testing laboratories while importing CRMs of heavy metals in rice.