Preventive and Therapeutic Effects of Total Flavonoids of Willow Buds on Acute Pancreatitis in Mice

To explore the preventive and therapeutic effects of total flavonoids of willow buds on acute pancreatitis is the objective of the study. The willow buds of Salix babylonica L. (similar to 1 cm) were harvested from Shenyang Institute of Technology between March 2020 to April 2020. Ultrasound-assisted extraction of total flavonoids of willow buds was conducted and an L-arginine-induced acute pancreatitis mouse model was established. The content of naringenin was determined by Agilent 1100 high-performance liquid chromatography. Serum biochemical test, serum oxidation test and plasma leukocyte content was determined and HE staining was performed. The protective and therapeutic effects of total flavonoids of willow buds on acute pancreatitis in mice were investigated. Total flavonoids of willow buds effectively decreased the activity of serum amylase, lipase and malondialdehyde, reduced plasma white blood cells and increased serum calcium content and superoxide dismutase activity in acute pancreatitis mice; total flavonoids of willow buds alleviated the pathological injury of the pancreas in acute pancreatitis mice. In conclusion, total flavonoids of willow buds may reduce pancreatic injury via reducing the inflammatory response and increasing the activity of antioxidant enzymes, which is a promising natural anti-acute pancreatitis drug.