Red Blood Cell Lysis Pretreatment Can Significantly Improve the Yield of Treponema pallidum DNA from Blood

Zi-Han Wei,Lin Xie,Yong-Jing Wang,Jiang-Xing Zhuang,Jian-Jun Niu,Li-Li Liu

Microbiology Spectrum（2023）

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摘要

Syphilis is a sexually transmitted disease caused by T. pallidum that can spread into the blood. T. pallidum DNA can be detected in blood by PCR but with low sensitivity. Few studies have applied RBC lysis pretreatment to blood T. pallidum DNA extraction.

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关键词

PCR, sample preparation, blood, blood component, DNA yield, Treponema pallidum, red blood cell lysis

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