Mp05-09 identification of aberrant glycosylation of osteopontin on urinary stone formation in urolithiasis patients and renal stone formation rats

You have accessJournal of UrologyCME1 Apr 2023MP05-09 IDENTIFICATION OF ABERRANT GLYCOSYLATION OF OSTEOPONTIN ON URINARY STONE FORMATION IN UROLITHIASIS PATIENTS AND RENAL STONE FORMATION RATS Go Anan, Tohru Yoneyama, Takuo Hirose, Makoto Sato, Takefumi Mori, and Chikara Ohyama Go AnanGo Anan More articles by this author , Tohru YoneyamaTohru Yoneyama More articles by this author , Takuo HiroseTakuo Hirose More articles by this author , Makoto SatoMakoto Sato More articles by this author , Takefumi MoriTakefumi Mori More articles by this author , and Chikara OhyamaChikara Ohyama More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003216.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Osteopontin (OPN) is one of the most important components in the calcium stone matrix. However, the relationship between glycosylation of OPN and urinary stone formation remains unknown. This study aims to identify the aberrant glycosylation profile of OPN related to urolithiasis in urolithiasis patients and renal calcium oxalate stone formation rats. METHODS: We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN (uFL-OPN) levels in 110 urolithiasis patients and 157 healthy volunteers. Also, we used rats with ethylene glycol-induced renal stones to evaluate the aberrant glycosylation of OPN in renal tissue. The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting. The assays were evaluated using the area under the receiver operating characteristics curve to discriminate stone forming urolithiasis patients. RESULTS: In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/uFL-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p<0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity. The Lycopersicon esculentum lectin (LEL) analysis of uFL-OPN showed that uFL-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers. The rats study showed that the levels of LEL-reactive OPN that had a polyLacNAc structure were increased in the renal cortex, even though total OPN levels did not increase. CONCLUSIONS: The aberrant glycosylation of OPN occurs in urine of urolithiasis patients and in renal cortex of renal stone formation rats. The aberrant glycosylation of OPN might be a marker of accelerated renal stone formation. Source of Funding: none © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e47 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Go Anan More articles by this author Tohru Yoneyama More articles by this author Takuo Hirose More articles by this author Makoto Sato More articles by this author Takefumi Mori More articles by this author Chikara Ohyama More articles by this author Expand All Advertisement PDF downloadLoading ...