A kinetic-pump integrated Microphysiological system for homeostasis on cell culture environments

In this study, we developed a kinetic-pump integrated microphysiological system (MPS) that supplies nutrients to cultured cells without changing the culture medium, aiming to realize a culture environment in which drug concentration in MPS is continuous for novel pharmacokinetic evaluation. The nutrients dialysis chamber of the MPS is separated into upper and lower compartments by a dialysis membrane, and nutrients are supplied by diffusion due the difference in its concentration. To evaluate the function of the MPS, dialysis function evaluation experiments and cell culture experiments were conducted. The results showed that the difference between the molecular weight of the accumulated dialysis membrane fractions and the molecular weight of the substance in the device enabled permeation and retention of the substance between the dialysis chamber and the cell culture chamber, and that cells in the cell culture chamber could be cultured only by the nutrient supply from the dialysis chamber.