Supplementary Figures, Legends, and Code from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Legends and still images for Supplemental Videos 1-13. Figure S1 provides a schematic illustration and images of device fabrication and setup. Figure S2 provides still images that summarize all intravasation events. Figure S3 provides still images of additional representative non-intravasation events following tumor cell division at the ECM-vessel interface. Figure S4 summarizes circularity and width measurements for all intravasation and non-intravasation events displayed in Figures S2 and S3. Figure S5 reveals anchored processes left behind after tumor cell intravasation. Figure S6 depicts multi-day tumor cell interactions at the ECM-vessel interface. Figure S7 shows a lack of tumor stem cell markers expressed by MDA-MB-231. Custom ImageJ code provided for quantifying maximum width of traced tumor cells.