Supplementary Figure S6 from TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation

Supplementary Figure S6 MiR-424 is post-transcriptionally regulated in vitro. Primary and mature miR-424 levels, by qPCR, in (A) HMLE cells constitutively expressing SNAI1 and in (B) HMLER cells constitutively expressing TWIST1 or SNAI1, after passaging the cell lines for longer than 20 days. SEM shown, n{greater than or equal to}3. *p<0.05, **p<0.01. (A) Two-tailed unpaired t-test and (B) one-way ANOVA with Bonferroni post-test.