Figure S6. sh-mediated RASSF1A depletion in HBEC-3 cells. from RASSF1A Suppresses the Invasion and Metastatic Potential of Human Non–Small Cell Lung Cancer Cells by Inhibiting YAP Activation through the GEF-H1/RhoB Pathway

HBEC-3 cells were transfected with non-silencing shRNA (Control), shRASSF1A-777 and shRASSF1A-779. A) Changes in RASSF1A and Yap expression assessed by immunofluorescence (Red=RASSF1A, Green=Yap, Blue=DAPI) experiments. Scale bar represents 50 µm. B) The migration speed (µm/h) of transfected cells was determined by wound assay on collagen IV. Mitomycin C (1 µg/ml) was added to medium 12h before wounding. The invasion capacity was assessed using a BioCoat Matrigel Invasion Chamber. The relative invasion was normalized to that of the cells transfected with Control. C) Representative images of colony formation of transfected cells in soft agar. Scale bar represents 20 µm. For all of the histograms, error bars indicate the standard error of the mean (SEM) of at least three independent experiments. *p<0.05, **p<0.01 and ***p<0.001, using an ANOVA test followed by Dunnett's test.